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Training Course: Mouse Embryo and Spermatozoa Cryopreservation

20th October 2025, 8:30 am – 4:30 pm

Cryopreservation allows long-term storage of embryos and sperm, providing a convenient way to preserve, protect and transport mouse lines for use in research. Archiving scientifically relevant mouse lines also adheres to good animal welfare practices by removing the need for continued animal breeding and is integral to any comprehensive colony management programme.

Running bi-annually since 2004, this course provides practical experience in murine embryo and spermatozoa freezing techniques routinely used at MRC Harwell and a simple, robust in vitro fertilisation procedure. The course will also cover the basic laboratory skills required when working in as assisted reproductive biology environment such as pipetting, weighing, microscope use, and embryo handling.

Date: Monday 20th – Thursday 23rd October 2025

Time: 08:30 – 16:30

Cost: £1,800.00 (cost is inclusive of accommodation however, other pricing options are available)

After this course, you’ll be able to:

  • Practical experience of sperm harvesting and freezing/thawing.
  • A clear understanding to set up an in vitro fertilisation session using both frozen and freshly harvested sperm.
  • Practical experience of oocyte and embryo harvesting for in vitro fertilisation and embryo freezing respectively.
  • Practical experience of embryo cryopreservation/thawing using a simple vitrification method.
  • An understanding of surgical embryo transfer, including some practical experience of pre-operative aseptic techniques and sub-cuticular suturing.
  • Practical experience of non-surgical embryo transfer techniques.
  • An understanding of the current methods used for handling and shipping samples and managing a cryo-archive, as well as an overview of blastocyst genotyping.

This course is designed for Researchers, Senior Animal Technicians and PhD Students.